Engineering the activity of amine dehydrogenase in the asymmetric reductive amination of hydroxyl ketones-Reference-Cited by-同舟云学术

Engineering the activity of amine dehydrogenase in the asymmetric reductive amination of hydroxyl ketones

Published:2022 Issue:19 Volume:12 Page:5952-5960
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Ming Hui¹²,Yuan Bo²³^ORCID,Qu Ge²³^ORCID,Sun Zhoutong²³^ORCID

Affiliation:

1. Department of Life Sciences and Medicine, University of Science and technology of China, Hefei 230022, China

2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China

3. National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China

Abstract

An engineered AmDH derived from a leucine dehydrogenase was used as the starting enzyme to improve its activity in the synthesis of (R)-3-amino-1-butanol. Preparative-scale synthesis of the (R)-product (90% yield, >99%) was performed on a gram-scale.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Natural Science Foundation of Tianjin City

Youth Innovation Promotion Association

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CY/D2CY00391K

Reference65 articles.