A substrate-binding-state mimic of H2O2-dependent cytochrome P450 produced by one-point mutagenesis and peroxygenation of non-native substrates-Reference-Cited by-同舟云学术

A substrate-binding-state mimic of H2O2-dependent cytochrome P450 produced by one-point mutagenesis and peroxygenation of non-native substrates

Published:2016 Issue:15 Volume:6 Page:5806-5811
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Shoji Osami¹²³⁴⁵,Fujishiro Takashi¹²³⁴⁵,Nishio Kousuke¹²³⁴⁵,Kano Yukiko¹²³⁴⁵,Kimoto Hiroshi¹²³⁴⁵,Chien Shih-Cheng¹²³⁴⁵,Onoda Hiroki¹²³⁴⁵,Muramatsu Atsushi¹²³⁴⁵,Tanaka Shota¹²³⁴⁵,Hori Ayumi¹²³⁴⁵,Sugimoto Hiroshi⁶⁷⁸⁵,Shiro Yoshitsugu⁶⁷⁸⁵,Watanabe Yoshihito⁹³⁴⁵

Affiliation:

1. Department of Chemistry

2. Graduate School of Science

3. Nagoya University

4. Nagoya 464-8602

5. Japan

6. RIKEN SPring-8 Center

7. Harima Institute

8. Sayo

9. Research Center for Materials Science

Abstract

H₂O₂-dependent cytochrome P450s that can catalyze monooxygenation of nonnative substrates were constructed by one-point mutagenesis.