Directed evolution of a cyclodipeptide synthase with new activities <i>via</i> label-free mass spectrometric screening-Reference-Cited by-同舟云学术

Directed evolution of a cyclodipeptide synthase with new activities via label-free mass spectrometric screening

Published:2022 Issue:25 Volume:13 Page:7581-7586
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Zhang Songya¹,Zhu Jing¹,Fan Shuai²,Xie Wenhao¹,Yang Zhaoyong²,Si Tong¹^ORCID

Affiliation:

1. CAS Key Lib Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

2. The Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 1000050, China

Abstract

A robotic workflow for directed evolution of new enzymatic activities via high-throughput library creation and label-free MS screening.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SC/D2SC01637K

Reference43 articles.

1. Directed Evolution: Bringing New Chemistry to Life

2. Directed Evolution: Methodologies and Applications

3. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

4. Directed evolution of glycosyltransferase for enhanced efficiency of avermectin glucosylation

5. Functional expression and stabilization of horseradish peroxidase by directed evolution inSaccharomyces cerevisiae

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