Efficient polyethylene terephthalate biodegradation by an engineered <i>Ideonella sakaiensis</i> PETase with a fixed substrate-binding W156 residue-Reference-Cited by-同舟云学术

Efficient polyethylene terephthalate biodegradation by an engineered Ideonella sakaiensis PETase with a fixed substrate-binding W156 residue

Published:2024 Issue: Volume: Page:
ISSN:1463-9262
Container-title:Green Chemistry
language:en
Short-container-title:Green Chem.

Author:

Yin Qingdian¹^ORCID,Zhang Jiaxing¹^ORCID,Ma Sen²,Gu Tao¹,Wang Mengfan²^ORCID,You Shengping¹³,Ye Sheng²,Su Rongxin¹⁴⁵³^ORCID,Wang Yaxin²,Qi Wei¹⁴⁵³^ORCID

Affiliation:

1. Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China

2. Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, P.R. China

3. Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, PR China

4. State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300350, PR China

5. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, PR. China

Abstract

The S92P/D157A variant of Ideonella sakaiensis PETase (IsPETase) showed significantly enhanced thermostability and PET degradation activity. The W156 residue of the variant was fixed in the substrate-binding conformation.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Pollution,Environmental Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2024/GC/D3GC03663D

Reference55 articles.

1. Enzymes’ Power for Plastics Degradation

2. Micro- and nanoplastics released from biodegradable and conventional plastics during degradation: Formation, aging factors, and toxicity

3. Plastics tsunami: Can a landmark treaty stop waste from choking the oceans?