Bioluminescent detection of viral surface proteins using branched multivalent protein switches-Reference-Cited by-同舟云学术

Bioluminescent detection of viral surface proteins using branched multivalent protein switches

Published:2024 Issue: Volume: Page:
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Gräwe Alexander¹^ORCID,Spruit Cindy M.²^ORCID,de Vries Robert P.²^ORCID,Merkx Maarten¹^ORCID

Affiliation:

1. Laboratory of Protein Engineering, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands

2. Utrecht Institute for Pharmaceutical Sciences, Department of Chemical Biology and Drug Discovery, Utrecht, The Netherlands

Abstract

Modular virus diagnostics is key to prevent virus spread. Here, we combine state-of-the-art synthetic biology tools to create a modular bioluminescent sensor platform of branched, multivalent protein switches for detecting viral surface proteins.

Funder

H2020 Marie Skłodowska-Curie Actions

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CB/D3CB00164D

Reference45 articles.

1. Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement

2. Lateral flow test engineering and lessons learned from COVID-19

3. Engineering Protein Switches for Rapid Diagnostic Tests

4. Glow-in-the-Dark Infectious Disease Diagnostics Using CRISPR-Cas9-Based Split Luciferase Complementation

5. Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice