One-step light-up metabolic probes for in situ discrimination and killing of intracellular bacteria

Author:

Yu Eric Y.1,Lee Michelle M. S.1,Chau Joe H. C.1,Lam Kristy W. K.1,Park Hojeong1ORCID,Kwok Ryan T. K.12,Lam Jacky W. Y.12,Li Yuanyuan23,Tang Ben Zhong1245ORCID

Affiliation:

1. Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, State Key Laboratory of Neuroscience, and Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, 999077, Hong Kong, China

2. HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st Rd, South Area Hi-Tech Park, Nanshan, Shenzhen 518057, China

3. College of Veterinary Medicine, Jilin University, Changchun 130062, China

4. Shenzhen Institute of Aggregate Science and Technology, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, 2001 Longxiang Boulevard, Longgang District, Shenzhen City, Guangdong 518172, China

5. State Key Laboratory of Luminescent Materials and Devices, and Center for Aggregation-Induced Emission (Guangzhou International Campus), South China University of Technology, Guangzhou 510640, China

Abstract

TPEPy-Ala and TPAPy-Kdo with metabolic moieties can be directly incorporated into the bacterial cell envelopes and light up intracellular bacteria. Additionally, the metabolic probes can effectively eliminate labeled bacteria in situ with minimal host cell cytotoxicity via photodyanmic therapy.

Funder

Research Grants Council, University Grants Committee

Innovation and Technology Commission

National Key Research and Development Program of China

Science and Technology Planning Project of Shenzhen Municipality

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,General Materials Science

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