Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1-Reference-Cited by-同舟云学术

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

Published:2020 Issue:14 Volume:10 Page:8181-8189
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Li Zhen¹²³⁴⁵^ORCID,Hu Bo¹²³⁴⁵,Zhou Rong¹²³⁴⁵^ORCID,Zhang Xiaojuan¹²³⁴⁵,Wang Ruizhe⁶⁷⁸⁹⁵,Gao Yun¹²³⁴⁵,Sun Mingjuan¹²³⁴⁵,Jiao Binghua¹²³⁴⁵,Wang Lianghua¹²³⁴⁵

Affiliation:

1. Department of Biochemistry and Molecular Biology

2. College of Basic Medical Sciences

3. Navy Medical University

4. Shanghai 200433

5. P. R. China

6. Spine Center

7. Department of Orthopedics

8. Changzheng Hospital Affiliated to Second Military Medical University

9. Shanghai

Abstract

For the first time, the aptamer of dinophysistoxin-1 was successfully obtained with high affinity and specificity by SELEX, and an aptasensor with a detection range from 40 to 600 nM was developed by biolayer interferometry.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/RA/C9RA10600F

Reference66 articles.

1. Occurrence of a new type of shellfish poisoning in the Tohoku district.

2. Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

3. Characterization of the dinophysistoxin-2 acute oral toxicity in mice to define the Toxicity Equivalency Factor