Role of detergents and nuclease inhibitors in the extraction of RNA from eukaryotic cells in complex matrices-Reference-Cited by-同舟云学术

Role of detergents and nuclease inhibitors in the extraction of RNA from eukaryotic cells in complex matrices

Published:2022 Issue:33 Volume:14 Page:12153-12161
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Holohan Cian¹^ORCID,Feely Nathan¹,Li Peng²,Curran Gerard²,Lee Gil U.¹^ORCID

Affiliation:

1. Conway Institute for Biomedical Research and School of Chemistry, University College Dublin, Ireland

2. Magnostics Ltd, 2 Clifton Lane, Monkstown, Co Dublin, Ireland

Abstract

Surfactants have a minimal impact on cell lysis in chaotropic lysis buffers, with the chaotropic agent sufficiently lysing cells in the absence of surfactant. Endogenous RNases negatively impacted qRT-PCR but were inactivated by the lysis buffer.

Funder

Science Foundation Ireland

Higher Education Authority

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2022/NR/D2NR02850F

Reference28 articles.

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