Discovery of a pyruvylated peptide-metabolizing enzyme using a fluorescent substrate-based protein discovery technique

Author:

Yoshioka Kentaro1234,Komatsu Toru12345,Hanaoka Kenjiro1234,Ueno Tasuku1234,Terai Takuya1234,Nagano Tetsuo6234,Urano Yasuteru12347

Affiliation:

1. Graduate School of Pharmaceutical Sciences

2. The University of Tokyo

3. Bunkyo-ku

4. Japan

5. Precursory Research for Embryonic Science and Technology (PRESTO)

6. Drug Discovery Initiative

7. Graduate School of Medicine

Abstract

We employed a fluorescent substrate-based target discovery approach to characterize an acylamino acid-releasing enzyme (APEH) as a pyruvylated peptide-metabolizing enzyme.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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