A new urinary exosome enrichment method by a combination of ultrafiltration and TiO2 nanoparticles-Reference-Cited by-同舟云学术

A new urinary exosome enrichment method by a combination of ultrafiltration and TiO2 nanoparticles

Published:2021 Issue:13 Volume:13 Page:1591-1600
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Xiang Xiaochao¹²³⁴⁵^ORCID,Guan Fulin¹²³⁴⁵,Jiao Fenglong¹²³⁴⁵^ORCID,Li Hang¹²³⁴⁵,Zhang Wanjun¹²³⁴⁵,Zhang Yangjun¹²³⁴⁵,Qin Weijie¹²³⁴⁵

Affiliation:

1. National Center for Protein Sciences Beijing

2. State Key Laboratory of Proteomics

3. Beijing Proteome Research Center

4. Beijing Institute of Lifeomics

5. Beijing 102206

Abstract

The workflow of separation and enrichment of exosomes by ultrafiltration–TiO₂. We proposed a new strategy for facile exosome isolation from human urine by utilizing the ultrafiltration technique and TiO₂, which can significantly reduce urine volumes, increase exposure of the material to exosomes in urine and obtain high purity exosomes.

Funder

National Natural Science Foundation of China

Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics

National Basic Research Program of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D1AY00102G

Reference34 articles.

1. A rapid, automated surface protein profiling of single circulating exosomes in human blood