Manipulating and probing enzymatic conformational fluctuations and enzyme–substrate interactions by single-molecule FRET-magnetic tweezers microscopy-Reference-Cited by-同舟云学术

Manipulating and probing enzymatic conformational fluctuations and enzyme–substrate interactions by single-molecule FRET-magnetic tweezers microscopy

Published:2014 Issue:26 Volume:16 Page:13052-13058
ISSN:1463-9076
Container-title:Phys. Chem. Chem. Phys.
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Guo Qing¹²³⁴,He Yufan¹²³⁴,Lu H. Peter¹²³⁴

Affiliation:

1. Bowling Green State University

2. Center for Photochemical Sciences

3. Department of Chemistry

4. Bowling Green, USA

Abstract

To investigate the critical role of the enzyme–substrate interactions in enzymatic reactions, the enzymatic conformation and enzyme–substrate interaction at a single-molecule level are manipulated by magnetic tweezers, and the impact of the manipulation on enzyme–substrate interactions are simultaneously probed by single-molecule FRET spectroscopy.

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2014/CP/C4CP01454E

Reference82 articles.

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5. H. P. Lu , Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium , 2010 , pp. 471–494

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