High-resolution probing heparan sulfate–antithrombin interaction on a single endothelial cell surface: single-molecule AFM studies-Reference-Cited by-同舟云学术

High-resolution probing heparan sulfate–antithrombin interaction on a single endothelial cell surface: single-molecule AFM studies

Published:2015 Issue:20 Volume:17 Page:13301-13306
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Guo Cunlan¹²³⁴⁵,Fan Xian⁶³⁴⁵,Qiu Hong⁶³⁴⁵,Xiao Wenyuan⁶³⁴⁵,Wang Lianchun⁶³⁴⁵,Xu Bingqian¹²³⁴⁵

Affiliation:

1. Single Molecule Study Laboratory

2. College of Engineering and Nanoscale Science and Engineering Center

3. University of Georgia

4. Athens

5. USA

6. Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology

Abstract

Using an antithrombin functionalized AFM probe, the specific interactions between antithrombin and heparan sulfate on endothelial cell surface were determined, revealing previously unexpected diverse binding sites as well as critical roles of N-, 2-O- and 6-O-sulfation in the interactions.

Funder

Division of Chemical, Bioengineering, Environmental, and Transport Systems

National Institutes of Health

Directorate for Biological Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2015/CP/C5CP01305D

Reference41 articles.

1. Heparan sulphate proteoglycans fine-tune mammalian physiology