Abstract
Introduction: Vanga is a dhatu, that has been used externally since the Samhita period. During the rasa shastra era, vanga was utilized internally for Prameha, (urinary disorder ) Medoroga(diseases of medadhatu – fat tis-sue), Kapha Vikaras(diseases of kapha-bodily constitution ), and other purposes after Bhasmikara-na.(preaparation of Bhasma - medicine ) Objective: Evaluate Acute and Sub-acute toxicity of Vanga Bhasma. Materials &Methods: The acute and sub-acute toxicity studies of Vanga Bhasma were carried out on Wistar Albino rats using 425 and 407 OECD guidelines, respectively. Acute oral toxicity study of the test drug was car-ried out at the limit dose of 2000 mg/kg orally in rats. In a sub-acute toxicity study, Vanga bhasma was adminis-tered to rats for 28 consecutive days at doses of 22.5(TED-therapeutic equivalent dose), 112.5(TEDX5), and 225 mg/kg(TEDX10). The effects of the drug were assessed on haematological, biochemical, histological, and pon-deral parameters. Results: Acute toxicity: the study showed the LD 50 value of Vanga Bhasma is greater than 2000mg/kg. Sub-acute toxicity: this study did not produce any signs or symptoms of toxicity at TED, TEDX5, and TEDX10. Conclusion: The single dose of 2000mg/kg body weight evaluated by acute toxicity study is proved to be non-toxic. Overall analysis reveals that test drug vanga bhasma is very well tolerated at rat human therapeutic equivalent dose and multiples of it. Therefore, Vanga bhasma, prepared and administered in accord-ance with customary protocols, is safe to consume at therapeutic dose levels.
Publisher
International Ayurvedic Medical Journal
Reference8 articles.
1. 1. Shree Vagbhatacharya, Rasa Ratna Samuchchaya Edited by Kaviraja Shree Ambikadatta Shastri, 8th Edn, Varanasi, Chaukhambha Amarabharati Publica-tion, 1988, 5th Chapter,162nd verse ,113pp.
2. 2. Sadhananda Sharma, Rasa Tarangini, Edited by Kasinatha Shastri, 11th Edn, Varanasi, Motilal Ba-narasi Das, 1979, 8th Taranga, 46 verse, 445pp.
3. 3. Paget GE, Barnes JM. Toxicity tests. In: Laurance DR, Bacharach AL, editors. Evaluation of drug activi-ties: pharmacometrics. New York: Academic Press; 1964. p. 205-10
4. 4. https://www.oecd.org/env/test-no-425-acute-oral-toxicity-up-and-down-procedure
5. 5. https://www.oecd.org/env/test-no-407-repeated-dose-28-day-oral-toxicity-study-in-rodents