Cryoprotective Effects of Longan Honey on Preantral Follicle Integrity of Rat Ovary Post Vitrification

Abstract

BACKGROUND: Longan honey (LH) has the potential as a natural extracellular cryoprotectant to maintain the integrity of intact preantral follicles against the cryoinjury during ovarian vitrification. OBJECTIVE: This research determined the cryoprotective effects of logan honey on preantral follicles integrity of rat ovary post-vitrification. MATERIALS AND METHODS: After vitrification, the follicle index was determined by observing the ovarian histological sections made using the paraffin method with hematoxylin-eosin staining and analyzed using Optilab 3.0 and Image Raster software. RESULTS: The results showed that the combination of ethylene glycol (EG) with LH and a dose variation of 7.5%-15% (KP1-KP4) increased the density of follicles, the number of intact follicles in G2 and G3, with a decrease in the atretic follicles in G1 better compared to the use of EG only (KKP1-KKP2). The differences in the histological structur e of preantral follicles affected the doses of LH needed by each type of follicle to maintain the integrity of the follicular structure from cryoinjury effects. The comparison of the G2 total follicle index values were KKP1 (90.7±18.3), KKP2 (115.6±9.9) < KP1 (193.6±10.7), KP2 (189.3±10.5), KP3 (182.2±27.1) and KP4 (169.4±8.8). Among the variations in the dose of LH 7.5%-15%, the highest increase in the G3 index value was found in primary (51.653±9.791), tertiary (43.119±8.786), secondary (33.885±4.745), and primordial (28.670±2.516) follicles of KP3 (7.5% of LH). CONCLUSION: The primary and tertiary follicular stages maintain the best integrity and can be used after the vitrification of rat ovaries.