THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY-Reference-Cited by-同舟云学术

THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY

Published:2022-09-28 Issue:3 Volume:7 Page:446-450
ISSN:2499-9962
Container-title:Russian Journal of Biological Physics and Chemisrty
language:en
Short-container-title:

Author:

Soloveva A.¹

Affiliation:

1. Privolzhsky Research Medical University

Abstract

Combined thermal trauma (CTT), including thermal inhalation exposure and burns of the skin, is often found in victims entering burn centers. The aim of the study was to study the peculiarities of regulation of antioxidant protection enzymes in the blood during experimental CTT under the influence of NO. The experiment was carried out on 30 white male rats of the Wistar line. The animals were divided into 3 groups of equal numbers: 1 – intact rats; 2 – control – animals with CTT; 3 – experimental – animals with CTT who received inhalation NO. Rats under anesthesia were subjected to contact burn (20% of the body surface) and thermal inhalation exposure. The animals were removed from the experiment after CTT for 10 days. The activity of superoxide dimsutase (SOD), catalase, glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (Gl-6-fDh) was determined in red blood cells. Inhalation-external exposure to NO during CTT was carried out daily for 10 days for 10 minutes. Kinetic parameters of the enzymatic reaction were calculated. Statistical processing of the results was carried out using Statistica 6.0 (Statsoft Inc., USA). It was shown that against the background of a decrease in the activity of antioxidant enzymes in the blood during CTT, the greatest inhibition was noted for catalase. The kinetic method of analysis for the first time established the nature of enzyme inhibition in CTT: for SOD and catalase in erythrocytes – two-parametrically consistent, for GR and Gl-6–fDh - catalytic. Inhalations of NO during CTT activated SOD, catalase, GR and Gl-6-fDh.

Publisher

RIOR Publishing Center

Reference19 articles.

1. Крылов К.М., Орлова О.В., Шлык И.В. Алгоритм действий по оказанию медицинской помощи пострадавшим с ожогами на догоспитальном этапе. Скорая медицинская помощь, 2010, № 2, с. 55-59., Krylov K.M., Orlova O.V., SHlyk I.V. The algorithm of actions for providing medical care to victims with burns at the pre-hospital stage. Skoraya medicinskaya pomoshch', 2010, no. 2, pp. 55-59. (In Russ.)

2. Глуткин А.В., Ковальчук В.И. Термический ожог кожи у детей раннего возраста (опыт эксперимента и клиники). Монография. Гродно: ГрГМУ, 2016, 180 с., Glutkin A.V., Koval'chuk V.I. Thermal skin burn in young children (experimental and clinical experience). Monograph. Grodno: GrGMU, 2016, 180 p. (In Russ.)

3. Шень Н.П. Ожоги у детей. М.: Триада-Х, 2011, 148 с., SHen' N.P. Burns in children. M.: Triada-H, 2011, 148 p. (In Russ.)

4. Mendonca Machado N., Gragnani A., Masako Ferreira L. Burns, metabolism and nutritional requirements. Nutr. Hosp., 2011, vol. 26, no. 4, pp. 692-700, doi: 10.1590/S0212-16112011000400005., Mendonca Machado N., Gragnani A., Masako Ferreira L. Burns, metabolism and nutritional requirements. Nutr. Hosp., 2011, vol. 26, no. 4, pp. 692-700, doi: 10.1590/S0212-16112011000400005.

5. Mokline A., Abdenneji A., Rahmani I. , Gharsallah L., Tlaili S., Harzallah I., Gasri B., Hamouda R., Messadi A.A. Lactate: prognostic biomarker in severely burned patients. Annals of Burns and Fire Disasters, 2017, vol. 30, no. 1, pp. 35-38., Mokline A., Abdenneji A., Rahmani I. , Gharsallah L., Tlaili S., Harzallah I., Gasri B., Hamouda R., Messadi A.A. Lactate: prognostic biomarker in severely burned patients. Annals of Burns and Fire Disasters, 2017, vol. 30, no. 1, pp. 35-38.