WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION
Author:
Nakvasina M.1, Artyukhov V.1, Starikova T.1, Saradzhi N.1
Affiliation:
1. Voronezh State University
Abstract
The changes in marker indicators of apoptotic cell death were studied: the level of functional activity of initiating caspases-8, -9, -12, membrane death receptors Fas, intracellular reactive oxygen species, cytosolic calcium, mitochondrial potential of peripheral blood lymphocytes of donors induced by exposure to hydrogen peroxide at final concentrations of 10-6 and 10-5 mol/l. Activation of initiating caspase-8 associated with membrane death receptors Fas was detected after exposure to hydrogen peroxide lymphocytes at a concentration of 10-6 mol/l. There was a significant increase in the functional activity of initiating caspase-9, the level of Fas receptors and intracellular reactive oxygen species, as well as a decrease in the concentration of cytosolic calcium and the value of the mitochondrial membrane potential 1 and 2 hours after modification of cells with hydrogen peroxide at a concentration of 10-5 mol/l compared with those for intact immunocytes. It was found that apoptosis of lymphocytes under the influence of exogenous hydrogen peroxide is realized with the participation of receptor-mediated caspase, mitochondrial (with activation of caspase-9) and p53-dependent mechanisms. The protective effect of resveratrol (10-6 mol/l) on lymphocytes after exposure to hydrogen peroxide (10-5 mol / l), which is due to its ability to reduce the level of reactive oxygen species in intact and modified hydrogen peroxide immunocytes, as well as deactivate hydrogen peroxide.
Publisher
RIOR Publishing Center
Reference19 articles.
1. Nagata S., Masato Tanaka. Programmed cell death and the immune system. Nature Reviews Immunology, 2017, vol. 17, no. 5, pp. 333-340, doi: 10.1038/nri.2016.153., Nagata S., Masato Tanaka. Programmed cell death and the immune system. Nature Reviews Immunology, 2017, vol. 17, no. 5, pp. 333-340, doi: 10.1038/nri.2016.153. 2. Savitskaya M.A., Onishchenko G.E. Mechanisms of Apoptosis. Biochemistry (Mosc.), 2015, vol. 80, no. 11, pp. 1393-1405, doi: 10.1134/S0006297915110012., Savitskaya M.A., Onishchenko G.E. Mechanisms of Apoptosis. Biochemistry (Mosc.), 2015, vol. 80, no. 11, pp. 1393-1405, doi: 10.1134/S0006297915110012. 3. Наквасина М.А., Артюхов В.Г. Механизмы клеточной гибели: апоптоз, аутофагия, некроз. Учебное пособие. Воронеж: Издательский дом ВГУ, 2019, 164 с., Nakvasina M.A., Artyukhov V.G. Mechanisms of cell death: apoptosis, autophagy, necrosis. Textbook. Voronezh: VSU Publishing House, 2019, 164 p. (In Russ.) 4. Mates J.M., Sanchez-Jimenez F.M. Role of reactive oxygen species in apoptosis: implications for cancer therapy. The International Jornal of Biochemistry & Cell Biology, 2000, vol. 32, pp. 157-170, doi: 10.1016/s1357-2725(99)00088-6., Mates J.M., Sanchez-Jimenez F.M. Role of reactive oxygen species in apoptosis: implications for cancer therapy. The International Jornal of Biochemistry & Cell Biology, 2000, vol. 32, pp. 157-170, doi: 10.1016/s1357-2725(99)00088-6. 5. Riazantseva N.V., Novitskii V.V., Chasovskikh N.Iu., Kaigorodova E.V., Starikova E.G., Starikov Iu.V., Radzivil T.T., Krat N.V. The role of redox-dependent signal systems in the regulation of apoptosis under oxidative stress condition. Tsitologiia, 2009, vol. 51, no. 4, pp. 329-334. (In Russ.), Riazantseva N.V., Novitskii V.V., Chasovskikh N.Iu., Kaigorodova E.V., Starikova E.G., Starikov Iu.V., Radzivil T.T., Krat N.V. The role of redox-dependent signal systems in the regulation of apoptosis under oxidative stress condition. Tsitologiia, 2009, vol. 51, no. 4, pp. 329-334. (In Russ.)
|
|