INFLUENCE OF SALMONELLA LIPOPOLYSACCHARIDE ON THE DIFFUSION OF TOLUIDINE BLUE IN ALGINATE HYDROGEL
Author:
Novakovskaya M.1, Ignatieva M.1, Cherenkov I.1
Affiliation:
1. Udmurt State University
Abstract
The paper considers the data of electrochemical experiments to study the diffusion of toluidine blue in an alginate hydrogel with the direct addition of lipopolysaccharide of different concentrations into the system. Using the method of cyclic voltammetry, changes in the strength of the current flowing through an electrochemical cell were recorded, on which a voltage varying over time was applied. The dependence of redox transformations of phenothiazine dye in model solutions of endotoxin was revealed. It has been shown that the presence of bacterial toxin in alginate hydrogel has a significant effect on the electrochemical behavior of the dye - in the range of lipopolysaccharide concentrations of 0.1-10 mcg/ml. Qualitative changes in comparison with the control sample were recorded on all received voltammograms. The introduced lipopolysaccharide isolated from Salmonella typhi bacteria contributed to an increase in both the anode and cathode currents, as well as a shift in potential values both when it was directly added to the cell and after 30 minutes of diffusion. Depending on the "current-time" for the recovery process, an increase in cathode peak values was observed with an increase in the concentration of endotoxin in the electrochemical system. The data obtained indicate the importance of taking into account the qualitative composition of the hydrogel medium, as well as the additional components introduced into it during the bioelectrochemical experiment.
Publisher
RIOR Publishing Center
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