APPLICATION OF MICROSPECTROFLUORIMETER/MICROSPECTROPHOTOMETER FOR THE STUDY OF THE SURFACE OF PLANT CELLS
Author:
Roshchina V.1, Kunyev A.1, Fateryga Valentina2, Shovkun M.3
Affiliation:
1. PSCBR RAS 2. A.O. Kovalevsky Institute of Biology of the South Seas of RAS 3. Prioksko-Terrasny State Nature Biosphere Reserve
Abstract
The fluorescence and absorbance spectra of intact plant leaf cells from juniper Juniperus excelsa, Jerusalem-sage Phlomis tuberosa, butterbur Petasites spurius, centaury Centaurea scabiosa and wild chervil Anthriscus sylvestris have been obtained using the microspectrofluorimeter/ microspectrophotometer MSF-15 (LOMO, St. Petersburg). The obtained spectra of autofluorescence and absorbance made it possible to test the composition of compounds outside the cell wall. This tool is able to detect the presence of secretions on the surface of intact cells, including secondary metabolites with the fluorescence maxima in the region of 530-550 nm, characteristic of phenolic compounds. In the absorbance spectra of leaves, maxima were found in the region of 580-620 nm, which is peculiar to blue pigments azulenes. The presence of azulenes on the surface of cells has been confirmed by short-term extraction of these pigments with ethanol or acetone. Similar methodical approach has been recommended for rapid analysis of intact surfaces of plant cells.
Publisher
RIOR Publishing Center
Reference9 articles.
1. Karnaukhov V.N., Yashin V.A., Kulakov V.I., Vershinin V.M., Dudarev V.V. Apparatus for investigation of fluorescence characteristics of microscopic objects. USA Patent, 1982, vol. 354, no. 4, iss. 114, pp. 1-14., Karnaukhov V.N., Yashin V.A., Kulakov V.I., Vershinin V.M., Dudarev V.V. Apparatus for investigation of fluorescence characteristics of microscopic objects. USA Patent, 1982, vol. 354, no. 4, iss. 114, pp. 1-14. 2. Karnaukhova N.A, Sergievich L.A., Karnaukhov V.N. Application of microspectral analysis to study intracellular metabolism in single cells and cell systems. Natural Science, 2010, vol. 2, no. 5, pp. 444-449, Karnaukhova N.A, Sergievich L.A., Karnaukhov V.N. Application of microspectral analysis to study intracellular metabolism in single cells and cell systems. Natural Science, 2010, vol. 2, no. 5, pp. 444-449 3. Рощина В.В., Яшин В.А., Куньев А.Р., Солтани Г.А., Призова Н.К., Хайбулаева Л.М. Присутствие азуленов на поверхности растительных клеток как тест на чувствительность к озону. Биологические мембраны, 2022, т. 39, № 1, с. 54-62, doi: 10.1134/S1990747822010081., Roshchina V.V., Yashin V.A., Kunyev A.R., Soltani G.A., Prizova N.K., Khaibulaeva L.M. The presence of azulenes on the surface of plant cells as a test for sensitivity to ozone. Biological Membranes, 2022, vol. 39, no. 1, pp. 54-62 (In Russ.). 4. Коновалов Д.А. Природные азулены. Растительные ресурсы, 1995, т. 31, № 1, с.101-130., Konovalov D.A. Natural azulenes. Plant Resources, 1995, vol. 31, no. 1, pp.101-130 (In Russ.). 5. Золотарев В.М. Применение дифференцирования в спектроскопии отражения. Оптика и Спектроскопия, 2012, т. 112, № 1, с. 150-154., Zolotarev V.M. Application of differentiation in reflection spectroscopy. Optics and Spectroscopy, 2012, vol. 112, no. 1, pp. 150-154 (In Russ.).
|
|