Affiliation:
1. Saint-Petersburg State University
2. Saint-Petersburg State Pediatric Medical University
3. Kirov Military Medical Academy
Abstract
The article is devoted to the study of redox regulation of transepithelial ion transport in osmoregulatory epithelium. The pharmacological analogue of oxidized glutathione, drug glutoxim®, has antioxidant and radioprotective properties and is widely used as an immunomodulator and cytoprotector in complex therapy of bacterial, viral and oncological diseases. Using voltage-clamp technique, the influence of non-steroidal anti-inflammatory drug meloxicam, an inhibitor of the cyclooxygenase pathway of arachidonic acid oxidation, on glutoxim effect on Na+ transport in frog Rana temporaria skin was investigated. It was shown for the first time that frog skin preincubation with meloxicam significantly reduces the stimulatory effect of glutoxim on Na+ transport. The data obtained indicate the involvement of cyclooxygenase pathway of arachidonic acid oxidation in the effect of glutoxim on Na+ transport in frog skin epithelium. The results also contribute to a more detailed understanding of molecular mechanisms of pharmacological effect of glutoxim and meloxicam, and may be useful for their application in clinics.
Reference21 articles.
1. Наточин Ю.В. Основы физиологии почки. Л.: Наука, 1982, 184 с., Natochin Yu.V. Fundamentals of kidney physiology. L.: Nauka, 1982, 184 p. (In Russ.).
2. Zhukov O.B., Zubarev A.R., Mezentseva M.V., Andryushkova Yu.A., Ose I.V. Modern aspects of immunomodulating therapy for those sick with recurrent infections of sexual transmission and with antibiotic-resistant bacterial prostatitis. Vracheb. Sosl., 2004, vol. 5/6, pp. 51-56., Zhukov O.B., Zubarev A.R., Mezentseva M.V., Andryushkova Yu.A., Ose I.V. Modern aspects of immunomodulating therapy for those sick with recurrent infections of sexual transmission and with antibiotic-resistant bacterial prostatitis. Vracheb. Sosl., 2004, vol. 5/6, pp. 51-56.
3. Effect of disulfide-containing compounds on the Na+ transport in the frog skin
4. Involvement of tyrosine and phosphatidylinositol kinases in oxidized glutathione and glutoxim regulation of Na+ transport in frog skin
5. Melnitskaya A.V., Krutetskaya Z.I., Lebedev O.E. The involvement of protein kinase C in the effect of oxidized glutathione and glutoxim on Na+ transport in frog skin. Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology, 2009, vol. 3, no. 3, pp. 323., Melnitskaya A.V., Krutetskaya Z.I., Lebedev O.E. The involvement of protein kinase C in the effect of oxidized glutathione and glutoxim on Na+ transport in frog skin. Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology, 2009, vol. 3, no. 3, pp. 323.