Crystallization and preliminary X-ray diffraction analysis of Gos1p, a yeast SNARE protein

Abstract

The Gos1 protein (Golgi SNAP receptor complex member 1) is involved in the SNARE complex, which is the core machinery that drives membrane fusion between cargo-carrying vesicles and their target membranes in the secretory and endocytic pathways in yeast. Truncated versions of the Gos1 protein fromSaccharomyces cerevisiaewere cloned, expressed, purified and crystallized. The crystal belonged to space groupP212121, with unit-cell parametersa= 39.67,b= 43.58,c= 81.94 Å, α = β = γ = 90°. An X-ray diffraction data set was collected at 100 K to 1.63 Å resolution. Matthews coefficient (VM) calculations suggest that one molecule is present in the asymmetric unit, corresponding to a solvent content of ∼55%.