Structures of prostaglandin F synthase from the protozoaLeishmania majorandTrypanosoma cruziwith NADP

Abstract

The crystal structures of prostaglandin F synthase (PGF) from bothLeishmania majorandTrypanosoma cruziwith and without their cofactor NADP have been determined to resolutions of 2.6 Å forT. cruziPGF, 1.25 Å forT. cruziPGF with NADP, 1.6 Å forL. majorPGF and 1.8 Å forL. majorPGF with NADP. These structures were determined by molecular replacement to a finalRfactor of less than 18.6% (Rfreeof less than 22.9%). PGF in the infectious protozoaL. majorandT. cruziis a potential therapeutic target.