Abstract
Small-animal physiology studies are typically complicated, but the level of complexity is greatly increased when performing live-animal X-ray imaging studies at synchrotron and compact light sources. This group has extensive experience in these types of studies at the SPring-8 and Australian synchrotrons, as well as the Munich Compact Light Source. These experimental settings produce unique challenges. Experiments are always performed in an isolated radiation enclosure not specifically designed for live-animal imaging. This requires equipment adapted to physiological monitoring and test-substance delivery, as well as shuttering to reduce the radiation dose. Experiment designs must also take into account the fixed location, size and orientation of the X-ray beam. This article describes the techniques developed to overcome the challenges involved in respiratory X-ray imaging of live animals at synchrotrons, now enabling increasingly sophisticated imaging protocols.
Funder
Centre for Advanced Laser Applications
Gottfried Wilhelm Leibniz program
National Health and Medical Research Council
Fay Fuller Foundation
Cure4CF Foundation
Robinson Research Institute Career Development Fellowship
Victorian Endowment for Science, Knowledge and Innovation
Technische Universität München, Institute for Advanced Study
Australian Synchrotron International Synchrotron Access Program
Publisher
International Union of Crystallography (IUCr)
Subject
Instrumentation,Nuclear and High Energy Physics,Radiation
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