Structural and microstructural interpretations of Zn-doped biocompatible bone-like carbonated hydroxyapatite synthesized by mechanical alloying

Abstract

Single-phase nanocrystalline biocompatible Zn-dopedA-type carbonated hydroxyapatite (A-cHAp) powder has been synthesizedviamechanical alloying of a stoichiometric mixture of CaCO3, CaHPO4·2H2O and ZnO powders in open air at room temperature by 10 h of milling. TheA-type carbonation in HAp (A-cHAp) is confirmed by Fourier transform IR analysis. The structural and microstructural parameters of the as-milled powders are revealed by Rietveld powder structure refinement analysis and transmission electron microscopy. Zn substitution along with mechanical alloying causes partial amorphization of crystallineA-cHAp, analogous to native bone mineral. Zn2+cations substitute into the ninefold-coordinated Ca2+sites in theA-cHAp unit cell. An assay test using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reveals a high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processedA-cHAp has a chemical composition very close to that of natural biological apatite.