Crystallization and preliminary diffraction studies of the RNA polymerase subunit RPB5 from Saccharomyces cerevisiae

Abstract

Crystals of the RNA polymerase subunit RPB5 from Saccharomyces cerevisiae have been obtained by vapour-diffusion techniques. The protein has been overexpressed in bacterial cells as a fusion with glutathione S-transferase. Two monoclinic crystal forms can be grown under different sets of conditions. In both cases, the diffraction is consistent with space group P21, with unit-cell parameters a = 45.3, b = 135.3, c = 47.3 Å, β = 118.6° for crystal form I and a = 48.4, b = 137.1, c = 47.1 Å, β = 118.6° for crystal form II.