The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

Abstract

Most macromolecular crystallography (MX) diffraction experiments at synchrotrons use a single-axis goniometer. This markedly contrasts with small-molecule crystallography, in which the majority of the diffraction data are collected using multi-axis goniometers. A novel miniaturized κ-goniometer head, the MK3, has been developed to allow macromolecular crystals to be aligned. It is available on the majority of the structural biology beamlines at the ESRF, as well as elsewhere. In addition, theStrategy for the Alignment of Crystals(STAC) software package has been developed to facilitate the use of the MK3 and other similar devices. Use of the MK3 andSTACis streamlined by their incorporation into online analysis tools such asEDNA. The current use ofSTACand MK3 on the MX beamlines at the ESRF is discussed. It is shown that the alignment of macromolecular crystals can result in improved diffraction data quality compared with data obtained from randomly aligned crystals.