Structure of a phosphoglycerate mutase:3-phosphoglyceric acid complex at 1.7 Å

Abstract

The crystal structure of the tetrameric glycolytic enzyme phosphoglycerate mutase from the yeast Saccharomyces cerevisiae has been determined to 1.7 Å resolution in complex with the sugar substrate. The difference map indicates that 3-phosphoglycerate is bound at the base of a 12 Å cleft, positioning C2 of the substrate within 3.5 Å of the primary catalytic residue, histidine 8.