Crystallization and X-ray diffraction studies of arginine kinase from the white Pacific shrimpLitopenaeus vannamei

Abstract

Crystals of an unligated monomeric arginine kinase from the Pacific whiteleg shrimpLitopenaeus vannamei(LvAK) were successfully obtained using the microbatch method. Crystallization conditions and preliminary X-ray diffraction analysis to 1.25 Å resolution are reported. Data were collected at 100 K on NSLS beamline X6A. The crystals belonged to space groupP212121, with unit-cell parametersa= 56.5,b= 70.2,c= 81.7 Å. One monomer per asymmetric unit was found, with a Matthews coefficient (VM) of 2.05 Å3 Da−1and 40% solvent content. Initial phases were determined by molecular replacement using a homology model ofLvAK as the search model. Refinement was performed withPHENIX, with finalRworkandRfreevalues of 0.15 and 0.19, respectively. Biological analysis of the structure is currently in progress.