Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F2αsynthase, AKR1B3

Abstract

Aldo-keto reductase 1B3 (AKR1B3) catalyzes the NADPH-dependent reduction of prostaglandin H2(PGH2), which is a common intermediate of various prostanoids, to form PGF2α. AKR1B3 also reduces PGH2to PGD2in the absence of NADPH. AKR1B3 produced inEscherichia coliwas crystallized in complex with NADPH by the sitting-drop vapour-diffusion method. The crystal was tetragonal, belonging to space groupP41212 orP43212, with unit-cell parametersa = b = 107.62,c= 120.76 Å. X-ray diffraction data were collected to 2.4 Å resolution at 100 K using a synchrotron-radiation source.