Author:
Zander Ulrich,Hoffmann Guillaume,Cornaciu Irina,Marquette Jean-Pierre,Papp Gergely,Landret Christophe,Seroul Gaël,Sinoir Jérémy,Röwer Martin,Felisaz Frank,Rodriguez-Puente Sonia,Mariaule Vincent,Murphy Peter,Mathieu Magali,Cipriani Florent,Márquez José Antonio
Abstract
Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting and processing largely depend on manual operations. Here, a series of new methods are presented based on the use of a low X-ray-background film as a crystallization support and a photoablation laser that enable the automation of major operations required for the preparation of crystals for X-ray diffraction experiments. In this approach, the controlled removal of the mother liquor before crystal mounting simplifies the cryocooling process, in many cases eliminating the use of cryoprotectant agents, while crystal-soaking experiments are performed through diffusion, precluding the need for repeated sample-recovery and transfer operations. Moreover, the high-precision laser enables new mounting strategies that are not accessible through other methods. This approach bridges an important gap in automation and can contribute to expanding the capabilities of modern macromolecular crystallography facilities.
Publisher
International Union of Crystallography (IUCr)
Cited by
73 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献