A complement to the modern crystallographer's toolbox: caged gadolinium complexes with versatile binding modes

Author:

Stelter Meike,Molina Rafael,Jeudy Sandra,Kahn Richard,Abergel Chantal,Hermoso Juan A.

Abstract

A set of seven caged gadolinium complexes were used as vectors for introducing the chelated Gd3+ion into protein crystals in order to provide strong anomalous scattering forde novophasing. The complexes contained multidentate ligand molecules with different functional groups to provide a panel of possible interactions with the protein. An exhaustive crystallographic analysis showed them to be nondisruptive to the diffraction quality of the prepared derivative crystals, and as many as 50% of the derivatives allowed the determination of accurate phases, leading to high-quality experimental electron-density maps. At least two successful derivatives were identified for all tested proteins. Structure refinement showed that the complexes bind to the protein surface or solvent-accessible cavities, involving hydrogen bonds, electrostatic and CH–π interactions, explaining their versatile binding modes. Their high phasing power, complementary binding modes and ease of use make them highly suitable as a heavy-atom screen for high-throughputde novostructure determination, in combination with the SAD method. They can also provide a reliable tool for the development of new methods such as serial femtosecond crystallography.

Publisher

International Union of Crystallography (IUCr)

Subject

General Medicine,Structural Biology

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