Crystallization of L-aspartate oxidase, the first enzyme in the bacterial de novo biosynthesis of NAD-Reference-Cited by-同舟云学术

Crystallization of L-aspartate oxidase, the first enzyme in the bacterial de novo biosynthesis of NAD

Published:1999-02-01 Issue:2 Volume:55 Page:549-551
ISSN:0907-4449
Container-title:Acta Crystallographica Section D Biological Crystallography
language:
Short-container-title:Acta Crystallogr D Biol Cryst

Author:

Bacchella Luca,Lina Claudio,Todone Flavia,Negri Armando,Tedeschi Gabriella,Ronchi Severino,Mattevi Andrea

Abstract

The flavoenzyme L-aspartate oxidase from Escherichia coli was crystallized using the hanging-drop vapour-diffusion technique with PEG 4000 as precipitant. The crystals belong to space group P3121 (or P3221) with unit-cell parameters a = b = 84.9, c = 159.9 Å. A solvent content of 42% corresponds to a monomer (60 kDa) in the asymmetric unit. A complete 2.8 Å resolution data set was collected using a rotating-anode X-ray generator.

Publisher

International Union of Crystallography (IUCr)

Subject

General Medicine,Structural Biology

Link

http://journals.iucr.org/d/issues/1999/02/00/li0305/li0305.pdf

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