Author:
Leung Adelaine K. W.,Park Michael M. V.,Borhani David W.
Abstract
Determination of the density of protein crystals by flotation in organic solvent density gradients using simple methods for the preparation of the density gradients and the transfer of crystals into these gradients is described. The method was tested with crystals of hen egg white lysozyme. These methods are especially suitable for use with fragile, high-solvent-content protein crystals. These methods were applied to the measurement of the density of human apolipoprotein A-I crystals.
Publisher
International Union of Crystallography (IUCr)
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
21 articles.
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