Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34

Abstract

The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726–1289) diffracting X-rays to 2.9 Å resolution, crystals of gp34(781–1289) diffracting to 1.9 Å resolution and crystals of gp34(894–1289) diffracting to 3.0 and 2.0 Å resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726–1289) and gp34(894–1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781–1289) and gp34(894–1289). For the latter, high-quality anomalous signal was obtained.