Comparative analysis of anti-polyglutamine Fab crystals grown on Earth and in microgravity
-
Published:2016-09-22
Issue:10
Volume:72
Page:762-771
-
ISSN:2053-230X
-
Container-title:Acta Crystallographica Section F Structural Biology Communications
-
language:
-
Short-container-title:Acta Cryst Sect F
Author:
Owens Gwen E.,New Danielle M.,Olvera Alejandra I.,Manzella Julia Ashlyn,Macon Brittney L.,Dunn Joshua C.,Cooper David A.,Rouleau Robyn L.,Connor Daniel S.,Bjorkman Pamela J.
Abstract
Huntington's disease is one of nine neurodegenerative diseases caused by a polyglutamine (polyQ)-repeat expansion. An anti-polyQ antigen-binding fragment, MW1 Fab, was crystallized both on Earth and on the International Space Station, a microgravity environment where convection is limited. Once the crystals returned to Earth, the number, size and morphology of all crystals were recorded, and X-ray data were collected from representative crystals. The results generally agreed with previous microgravity crystallization studies. On average, microgravity-grown crystals were 20% larger than control crystals grown on Earth, and microgravity-grown crystals had a slightly improved mosaicity (decreased by 0.03°) and diffraction resolution (decreased by 0.2 Å) compared with control crystals grown on Earth. However, the highest resolution and lowest mosaicity crystals were formed on Earth, and the highest-quality crystal overall was formed on Earth after return from microgravity.
Funder
National Institute of General Medical Sciences
Publisher
International Union of Crystallography (IUCr)
Subject
Condensed Matter Physics,Genetics,Biochemistry,Structural Biology,Biophysics
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献