Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα–BF2*0401 complex

Author:

Liu Yanjie,Chen Rong,Tariq Mansoor,Xia Chun

Abstract

In the process of antigen presentation, the MHCI–CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα–BF2*0401) are reported. This complex was verified by SDS–PAGE analysis of a CD8αα–BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401,β2-microglobulin and CD8α, respectively. The crystal of CD8αα–BF2*0401 diffracted to 2.8 Å resolution and belonged to space groupP21, with unit-cell parametersa= 90.6,b= 90.8,c= 94.9 Å,β= 98°. The Matthews coefficient and solvent content were calculated to be 2.88 Å3 Da−1and ∼57.3%, respectively.

Publisher

International Union of Crystallography (IUCr)

Subject

Condensed Matter Physics,Genetics,Biochemistry,Structural Biology,Biophysics

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