Effect of Biochanin-A on The Cerebellum in Cerebral Ischemia Reperfusion Injury

Abstract

ABSTRACT Objective: To examine the protective effect of Biochanin A (BCA) on the cerebellum in cerebral ischemia reperfusion injury. Material and Metodhs: 24 Wistar albino female rats were divided into 3 groups. Control, Ischemia reperfusion (IR), IR +BCA (20 mg/kg intraperitoneal) group. A micro bulldog clamp was placed on the left common carotid artery of the rats and cerebral ischemia was applied for 2 hours. After cerebral ischemia, the clamp was removed and reperfusion was performed for 24 hours. After 7 days, all rats were decapitated and the protective effects of Biochanin A on the cerebellum were evaluated by immunohistochemically analyses. Results: In the IR group; S100 expression was also observed positive in neurons and neuroglia in the pia mater, molecular layer, Purkinje cells in the ganglionic layer and granule cells in the granular layer, and neuroglia in the white matter. In the IR+ Biochanin A group, it was observed that the immune activity in the granule cells and Purkinje cells in the granular layer was reduced compared to the IR group. Conclusion: We suggest that Biochanin A treatment has a potential therapeutic role in alleviating inflammation in the cerebellum after cerebral ischemia reperfusion.