Validation of the Multiresidue Method Analysis for Pesticides in Bee Honey by UPLC-MS/MS using the Method of Samples Preparation QuEChERS

Abstract

In this paper we present the results of evaluating the suitability of the developed method for the simultaneous determination of 200 pesticide residues in bee honey using ultraperformance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS). The main stages of extraction of pesticides from the matrix using QuEChERS (Quick Easy Cheap Effective Rugged Safe) sample preparation are described. Appropriate mobile phase composition was used to better identify analytes, internal standards were not applied. The studied list of pesticides is relevant for use in modern agriculture. The limit of quantification of the method (LOQ) for each of the analytes is 0.001 mg/kg, the relative standard deviation in the conditions of repeatability and reproducibility < 20.0 %, the expanded measurement uncertainty is less than the limit of regulatory requirements < 50.0 %. The calibration graph for each of the analytes had a coefficient R2 > 0.99. The use of QuEChERS sample preparation methods allows to reduce labor intensity, time and reagents, increase the accuracy and precision of research in comparison with classical methods of sample preparation. The described method makes it possible to detect a wide range of pesticides and has a fairly high sensitivity