Voltammetric bienzymatic sensor for sucrose determination in honey

Abstract

An electrochemical sensor based on nanostructured planar carbon electrodes (nanoSPCE) modified with a SiO2 biocomposite film containing MnO2 particles and enzymes glucose oxidase and invertase was developed. MnO2 particles obtained by electrochemical deposition possessed electrocatalytic activity toward hydrogen peroxide. Glucose oxidase (GOx) and invertase (Inv) were encapsulated into SiO2 film by method of electroassisted deposition. The electrode modified with biocomposite film nanoSPCE/MnO2/GoX/Inv/SiO2 was electroactive toward sucrose. The indicator reaction was oxidation of H2O2 – the product of bienzymatic reaction which was catalyzed by MnO2 on the electrode surface. The linearity range of the calibration graph for the voltametric determination of sucrose using developed modified electrode is 0.017-0.342 mg/ml, the detection limit is 0.006 mg/ml. The obtained nanoSPCE/MnO2/GoX/Inv/SiO2 electrode demonstrated satisfactory stability of the analytical signal during one month of operation. The developed method was used for sucrose determination in the honey samples. The 50-fold molar excess of glucose and fructose does not interfere the determination of sucrose.