ANTIHERPETIC ACTION OF CERIUM SALTS IN VITRO

Abstract

Compounds based on cerium are highly promising objects in biotechnology regarding their high biological activities such as antiviral, antibacterial, antifungal, neuro- and radioprotective action, and antioxidant activity. On their basis is possible to develop compositions capable of activating the systems of cellular and humoral immune defence and use them for the prevention and therapy of viral diseases, which makes it achievable to use them for the development of potential antiherpetic agents. Despite the success of their application in biotechnological fields, the mechanism of their action on biological objects requires detailed research. The work aimed to verify in vitro anti-HSV-1/2 activity of trivalent and tetravalent cerium salts (1 mM – 0.01 nM) according to the preventive and therapeutic regimen. Methods: virological, cytological, statistical. Results. The therapeutic regime was noneffective. In the preventive regime, salt (NH4)2Ce(NO3)6 in vitro forms antiviral resistance in the range of investigated concentrations, while the salt CeCl3·7Н2О forms a non-linear, sinusoidal-like concentration-dependent anti-HSV-1/2 response of cells. Conclusions. Cerium salts (III and IV) can cause the formation of a state of antiviral resistance in the model system MA-104 - HSV-1/2 during their previous 24 h of contact with test cells. Cerium salt (IV) provides 50% inhibition of the cytopathic action of HSV-1/2 at a concentration of 1 μM. It is assumed that the shown antiviral activity of cerium salts may be due to their effect on the interferon system and the formation of antiviral resistance in cells.