CYTOTOXIC / CYTOSTATIC EFFECT OF CULTIVATED MUSHROOMS FLAMMULINA VELUTIPES, CYCLOCYBE AEGERITA AND HERICIUM ERINACEUS

Abstract

The use in clinical and pharmaceutical practice of biological preparations obtained from natural raw materials, in particular from mushrooms, is of urgent importance. Currently, the cultivation technologies of medicinal mushrooms are widely used, the mycelium of which contains a significant amount of cytokinins – stimulators of proliferation and differentiation of various types of plant cells, which also exhibit an immunomodulatory and oncostatic effects in the organism of animals and humans. Therefore, the aim of this work was to determine the cytotoxic/cytostatic effect of cultivated mushrooms Flammulina velutipes, Cyclocybe aegerita and Hericium erinaceus. Purification of cytokinins was carried out in stages, using methods of centrifugation, fractionation with n-butanol, ion exchange chromatography on Dowex 50Wx8 columns and thin-layer chromatography on Silicagel 60 F254. Identification and quantification of cytokinins was performed by by liquid chromatography in the reverse phase of MS (Agilent 1200 LC, USA) on an Agilent Zorbax Eclipse XDB-C18 column. Cytotoxic/cytostatic screening was performed on HeLa cells derived from human cervical cancer tumors using the MTT assay and routine counting of the ratio of live to dead cells. The results of the research showed that the highest content of cytokinins per 1 g of dry weight was characteristic of cultivated mushrooms of the species H. erinaceus. Also, H. erinaceus has the highest toxicity against human cervical cancer cells of the HeLa line, while the mushrooms F. velutipes and C. aegerita showed a cytostatic (antiproliferative) effect.