The influence of cadmium and essential nanometals on the physiological And biochemical parameters of lettuce plants

Abstract

Due to the aim the effect of pre-sowing treatment with a nonionic colloidal solution of the mix of copper, zinc, manganese and iron nanoparticles, cadmium ions and their combined action on the physiological and biochemical indices: the content of lipid peroxidation products and balance of photosynthetic pigments in green Lolo and red Lolo Ross varieties of Lactuca sativa L. was investigated. Spectrophotometric methods of investigating the content of peroxides, thiobarbiturate-active products (with use of the modified Kumar and Knowles method), as well as the content of the main photosynthetic pigments (chlorophyll a, b, a/b ratio, and the carotenoid content) with the Wintermans, de Mots calculations application were used. Due to the results, a decrease in the content of peroxides and growth of malonic dialdehyde was observed for most treatment variants of Lolo. The opposite tendency was observed for the Ross variety (the initial growth of these parameters under the nanoparticles influence decreased to the control during the fourteen-day exposure). The decrease of chlorophyll content was observed In the Lolo variety under the essential metal nanoparticles influence, in contrast to the action of cadmium.For the Ross variety, a significant reduction of both chlorophyll and carotenoid contents in all treatment types during exposure was calculated.The a/b-index for Lolo plants has significantly increased in variants of cadmium influence, for Ross plants the divergence of the influence of all treatment variants has been marked. As a result, the treatment with a mixture of essential nanometals showed additive stress-loading functions. It was observed in a growth of the level of lipid peroxidation products and the reduction of the photosynthetic pigment biomarkers in both varieties during the 0,1 mM cadmium exposure. The Lolo Ross variety showed a higher variability of results under different treatment, compared to the Lolo variety.