Influence of methyure and ivin preparations on vacuolar Ca2+/H+ antiporter of Zea mays L. root cells under salt stress

Abstract

Because calcium plays an important role in the regulation of growth and development processes in plant organism as well as the formation of a specific physiological response to the action of various stressors, acting as a secondary messenger, the purpose of the study was to study the effect of heterocyclic drugs Methyure and Ivin of root cells Zea mays L. under salt stress. The roots of maize hybrids of Ostrich CB were exposed in the presence of 0.1 M sodium chloride for 1 and 10 days. Vacuolar membranes were obtained from corn root homogenate by centrifugation in a sucrose step gradient. The activity of the Са2+/Н+-antiporter was evaluated by the proton release from vesicles by adding calcium ions to the incubation medium, expressing ∆F% per mg of protein per minute. Under control conditions, heterocyclic drugs Methyure and Ivin do not affect the activity of Са2+/Н+-antiporter in the vacuolar membranes of corn roots and do not significantly contribute to the development of stress response in plants. At the same time, under the conditions of salt exposure of maize seedlings the opposite directed effect of Methyure and Ivin on the activity of Са2+/Н+-antiporter in the membranes of root vacuoles was revealed. Thus, under prolonged salt exposure, Methyure increases the activity of Са2+/Н+-antiporter in the vacuolar membrane of the root and, on the contrary, Ivin reduces it, which may be due to the differences in chemical composition of these heterocyclic drugs. The results, that were obtained, indicate that the salt-protective effect of the heterocyclic drug Methyure may also be due to its effect on the functional activity of the Са2+/Н+-antiporter in the vacuolar membrane of corn root.