Purification of microalgae crops of ACKU collection from fungal contaminants

Abstract

The work is focused to the selection of the best purification methods of microalgae strains from ACKU collection (Algae Culture Collection of Kyiv University) from contamination by microscopic fungi. The screening of microalgae culture collection ACKU (Algae Culture Collection of Kyiv University) is deal. Contamination of some microalgae strains by fungi of Cladosporium Link, Alternaria Nees, and Monilia Bonord genera was detected. The following strains were selected for the experiment: ACKU 139-02 (Klebsormidium flaccidum (Kütz.) PC Silva, Mattox et Blackwell), ACKU 293-04 (Acutodesmus obliquus (Turpin) P. Tsarenko), ACKU 364-04 (cf. Chlorosarcinopsis dissociata Herndon), ACKU 599-06 (Klebsormidium nitens Menegh. in Kützing), ACKU 600-06 (Klebsormidium flaccidum (Kütz.) PC Silva, Mattox et Blackwell) и ACKU 1056 (Desmodesmus abundans (Kirchn.) E. Hegew.). The effect of carbendazim (Methylbenzimidazol-2-ylcarbamate), Antibiotic Antimycotic Solution (penicillin – 10,000 IU, streptomycin – 10 mg, amphotericin B – 25 μg) and Nuosept BMc 422 on fungi-contaminants of green algal culture strains with different morphological structure (coccoid and filamentous) was studied. Experimental concentrations of substances: carbendazim – 0.005%, Antibiotic Antimycotic Solution – 1%, Nuosept BMc 422 – 0.05% and 0.2%. Was shown that Antibiotic-Antimycotic Solution and Nuosept BMc-422 at the selected concentrations were not effective for purifying of green algae strains from significant contamination by microscopic fungi. It was found that the carbendazim solution is effective in the purification of cocoid green algae strains from contamination by microscopic fungi Cladosporium cladosporioides (Fresen.) G.A. de Vries). As a result, an axenic culture was obtained for the ACKU strain No.293-04 (A. obliquus).