Subpopulation composition of blood T-killers in patients with hepatitis C with genotype 1 or 3-Reference-Cited by-同舟云学术

Subpopulation composition of blood T-killers in patients with hepatitis C with genotype 1 or 3

Published:2023-05-25 Issue:8 Volume: Page:142-149
ISSN:2658-5790
Container-title:Meditsinskiy sovet = Medical Council
language:
Short-container-title:Medicinskij sovet

Author:

Cherepnin M. A.¹,Tsukanov V. V.¹^ORCID,Savchenko A. A.¹^ORCID,Vasyutin A. V.¹^ORCID,Borisov A. G.¹^ORCID,Belenyuk V. D.¹^ORCID,Tonkikh J. L.¹^ORCID

Affiliation:

1. Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences”, Separate Subdivision “Scientific Research Institute of medical problems of the North”

Abstract

Introduction. Despite great attention to the pathogenesis of chronic viral hepatitis C (CVHC), many aspects of the immune response in this pathology remain unclear. Aim. To study the subpopulation composition of blood cytotoxic T cells by flow cytometry, depending on the severity of clinical and morphological manifestations of CVHC with genotype 1 or 3. Materials and methods. Clinical, laboratory examinations, determination of liver fibrosis by elastometry using the METAVIR scale and study of the subpopulation composition of cytotoxic T cells in the blood were carried out in 144 patients with CVHC, including 74 patients with genotype 1 and 70 individuals with genotype 3, and in 20 people of the control group. The study of the subpopulation composition of cytotoxic T cells in the blood was carried out on a flow cytometer Navios (Beckman Coulter, USA) with the determination of CD3, CD8, CD45R0 and CD62L markers. Results. Changes in the subpopulation composition of blood cytotoxic T cells were more associated with the severity of liver fibrosis in patients with 1 and 3 genotypes of CVHC, than with inflammatory activity and viral load. In patients with CVHC genotype 3, a marked decrease in the content of TEMRA T-cytotoxic cells (CD3+ CD8+ CD45R0–CD62L–) and effector memory T-cytotoxic cells (CD3+ CD8+ CD45R0+ CD62L–) was registered in patients with METAVIR liver fibrosis stage F3-F4 in comparison with persons with METAVIR liver fibrosis stage F0-F1 (Kruskal-Wallis test, respectively, p = 0.02 and p = 0.04 In persons with CVHC genotype 1, similar associations were expressed to a lesser extent.Conclusion. We obtained an association of deterioration in the indices of the blood cytotoxic T cells subpopulation in patients with CVHC with an increase in the severity of liver fibrosis, which had some differences in patients with genotypes 1 and 3.

Publisher

Remedium, Ltd.

Subject

General Medicine

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