Preliminary Quantitative Phytochemical, Physicochemical And In-Vitro Antioxidant Activity Of Senna Alata Hydroalcoholic Leaf Extract

Abstract

Many oxidative stress related diseases are as a result of accumulation of free radicals in the body. Present studies are directed towards finding natural antioxidants of plants origins. The present study was carried out to determine the phytochemical constituents, its qualities and antioxidant activity of Senna alata hydroalcoholic leaf extract as per the standard pharmacopoeial protocol. The aim of this study was to evaluate in vitro antioxidant activities and to screen for phytochemical constituents of Senna alata. The free radical scavenging activity of hydroalcoholic extract against various assay in terms of IC50 value were DPPH (2, 2- diphenyl-1-picrylhydrazyl) assay (715.26μg/ml), reducing power assay (715.26μg/ml), ABTS test (643.95μg/m) and Nitric oxide test (856.79μg / ml) was evaluated. The phytochemical analysis revealed the presence of secondary metabolites such as alkaloids, flavonoids, steroids, terpenoids, anthroquinone, phenols, saponin, tannin and carbohydrates. Quantitative phytochemical analysis revealed the amount of Alkaloid content (49.2%), Flavonoid content (6.96%) and Phenolic content (69.72%) present. Also the Physicochemical examination analysis were also carried out in Senna alata powdered sample which showed the presence of Total Ash (10.25 ± 0.04), Acid Insoluble Ash (2.75 ± 0.03), Water Soluble Ash (1.98 ± 0.02), Alcohol Extract Value (6.32 ± 0.03), Water Extract Value (11.19 ± 0.02), Loss on Dry (6.14 ± 0.03) and Foreign Matter (0.38 ± 0.01). Our findings provide evidence that the crude hydroalcoholic extract of S.alata is a potential source of natural antioxidants, and this justified its uses in folkloric medicines.