Influence of Short-Term Supplementation with Anabolic Steroid Drug Oxymetholone And /Or Creatine Widely Used In Ksa on Some Fertility Biomarkers in Albino Rats

Abstract

Abuse supplement is becoming a public health problem due to the adverse effects on health and semen quality in Saudi Arabia for the last few years. The present work is aimed to investigates the effect of individual supplementation with oxymetholone as Anabolic Androgenic Steroids (AAS) or creatine protein are the most common nutritional supplement use in the KSA and their mixture on some fertility biomarkers, oxidant-antioxidant activities as well as tissues structure of testis of albino rats. Forty adult male albino rats weighing around 150-200 g were classified into four groups of 10 rats each. Group I: control  untreated  group.  Group  II: treated  with  oxymetholone  (150  mg/day).  Group  III: treated  with creatine  protein  (20 mg/day). Group IV: treated with Oxymetholone (150 mg/day) and creatine protein (20 mg /day) simultaneously. All animals were given orally for 30 days. Individual supplementation with each of oxymetholone or creatine induced significant upregulation in oxidative stress biomarkers Malondialdehyde (MDA), Protein Carbonyls (PC), 8-Hydroxy Guanosine (8OHG). In addition, it also significantly  reduced  the  antioxidants  activities  Total  Antioxidant  Capacity  (TAC),  Catalase  (CAT),  Superoxide  Dismutase (SOD).  Remarkable  reduction  in  each  of  testosterone  hormone,  luteinizing  hormone,  sperm  counts,  and  motility  was pronounced in oxymetholone treated group than creatine group. The potentiality of oxymetholone was obvious in the mixture treated group. Changes in testis structure architecture follow the same previous trend that confirmed the clinical results. In conclusion; AAS and proteins should be used under supervision of special physicians because it will be very harmful for the reproductive life of users.