Role of miRNA 199a-5p Expression in Iraqi Women with Breast Cancer

Abstract

Background: Elevated levels of microRNAs have been linked to breast cancer and other types of cancer. It has been discovered that miRNA-199a-5p overexpression contributes to the epithelial-mesenchymal transition (EMT) in breast cancer. Objective: To examine the expression of microRNA 199a-5p in tissues from both benign and malignant breast cancers. Methods: We use 50 FFPE tissue samples divided into two groups based on their histology and cytological characteristics. The first group comprised 25 samples classified as benign, while the second group comprised 25 samples classified as malignant. To calculate the expression levels of miRNA-199a-5p, we use a real-time RT-PCR method based on SYBR green. Results: It was found that the folding value of miRNA-199a-5p in malignant tumors was 2.908 compared to benign tumors (2.027). However, the statistical significance of these differences was not significant. Conclusion: Depending on the specific context and molecular targets, miRNA-199a-5p plays a dual role in breast cancer development, acting as both an oncogene and a tumor suppressor.