High AAV vector purity results in serotype- and tissue-independent enhancement of transduction efficiency-Reference-Cited by-同舟云学术

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High AAV vector purity results in serotype- and tissue-independent enhancement of transduction efficiency

Published:2009-12-03 Issue:4 Volume:17 Page:503-510
ISSN:0969-7128
Container-title:Gene Therapy
language:en
Short-container-title:Gene Ther

Author:

Ayuso E,Mingozzi F,Montane J,Leon X,Anguela X M,Haurigot V,Edmonson S A,Africa L,Zhou S,High K A,Bosch F,Wright J F

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Molecular Biology,Molecular Medicine

Link

http://www.nature.com/articles/gt2009157.pdf

Reference29 articles.

1. Clark KR, Liu X, McGrath JP, Johnson PR . Highly purified recombinant adeno-associated virus vectors are biologically active and free of detectable helper and wild-type viruses. Hum Gene Ther 1999; 10: 1031–1039.

2. Davidoff AM, Ng CY, Sleep S, Gray J, Azam S, Zhao Y et al. Purification of recombinant adeno-associated virus type 8 vectors by ion exchange chromatography generates clinical grade vector stock. J Virol Methods 2004; 121: 209–215.

3. Gao G, Qu G, Burnham MS, Huang J, Chirmule N, Joshi B et al. Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo. Hum Gene Ther 2000; 11: 2079–2091.

4. Kaludov N, Handelman B, Chiorini JA . Scalable purification of adeno-associated virus type 2, 4, or 5 using ion-exchange chromatography. Hum Gene Ther 2002; 13: 1235–1243.

5. Qu G, Bahr-Davidson J, Prado J, Tai A, Cataniag F, McDonnell J et al. Separation of adeno-associated virus type 2 empty particles from genome containing vectors by anion-exchange column chromatography. J Virol Methods 2007; 140: 183–192.

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