Affiliation:
1. Division of Nuclear Medicine, Department of Radiology, University of Colorado Center for Health Sciences, Denver, Colorado, U.S.A.
Abstract
We have developed an autoradiographic method for estimating the oxidative and glycolytic components of local CMRglc(LCMRglc), using sequentially administered [18F]fIuorodeoxyglucose (FDG) and [14C]-6-glucose (GLC). FDG-6-phosphate accumulation is proportional to the rate of glucose phosphorylation, which occurs before the divergence of glycolytic ( GMg) and oxidative ( GMo) glucose metabolism and is therefore related to total cerebral glucose metabolism GMt: GMg+ GMo= GMt. With oxidative metabolism, the14C label of GLC is temporarily retained in Krebs cycle-related substrate pools. We hypothesize that with glycolytic metabolism, however, a significant fraction of the14C label is lost from the brain via lactate production and efflux from the brain. Thus, cerebral GLC metabolite concentration may be more closely related to GMothan to GMtIf true, the glycolytic metabolic rate will be related to the difference between FDG- and GLC-derived LCMRglc. Thus far, we have studied normal awake rats, rats with limbic activation induced by kainic acid (KA), and rats visually stimulated with 16-Hz flashes. In KA-treated rats, significant discordance between FDG and GLC accumulation, which we attribute to glycolysis, occurred only in activated limbic structures. In visually stimulated rats, significant discordance occurred only in the optic tectum.
Subject
Cardiology and Cardiovascular Medicine,Clinical Neurology,Neurology
Cited by
126 articles.
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