Transient Blood-Brain Barrier Permeability following Profound Temporary Global Ischaemia: An Experimental Study Using 14C-AIB

Abstract

The influence of reperfusion after profound incomplete forebrain ischaemia on blood–brain barrier (BBB) permeability to a small protein tracer was studied in male Sprague-Dawley rats, The mean cortical blood to brain transfer constant ( Ki) for 14C-amino isobutyric acid (AIB) was significantly greater at 3 and 6 h of reperfusion, 2.5 times the mean values of controls (p < 0.05) (2.5 μl g−1 min−1 and 1.0 μl g−1 min−1 respectively), but had returned to control values after reperfusion for 24 h. Analysis of distribution of Ki values showed that following 15 min and 30 min of profound ischaemia, there was a significant increase in transfer of AIB across the blood–brain barrier (BBB) after recirculation for up to 6 h, though there was no evidence of protein extravasation as assessed by Evans Blue (EB) dye. After 24 h of reperfusion, the BBB to AIB was restored, and Ki values had returned to control values. It is concluded that following transient global ischaemia, the BBB may recover rapidly.